美國野參抗癌靈 實驗報告

單獨使用植物精華（美國野參抗癌靈所含）對控制MCF-7乳腺癌細胞生長的效果

美國野參抗癌靈對控制癌細胞擴散的影響

Inhibition of Cell Proliferation (% of vehicle control)
	對控制癌細胞擴散的影響
Dose of Ginseng Extract	GS4	CHP&GS4
野參萃取物的劑量(mg/ml)	美國野山參單獨使用	美國野山參與植物精華共同使用
0.01毫克 / 毫升	100% (無效)	51% (將近一半的癌細胞被控制)
0.1毫克 / 毫升	85% (15%的癌細胞被控制)	35% (65%的癌細胞被控制)
1毫克 / 毫升	30% (70%的癌細胞被控制)	5% (95%的癌細胞被控制)

植物精華的劑量(毫克/毫升)

控制癌細胞生長的比例

單獨使用美國野山參(GS4)與美國野參抗癌靈(CHP) 對控制MCF-7癌細胞生長的效果比較

劑量(毫克/毫升)

控制癌細胞生長的比例

野山參萃取物對服有雌性荷爾蒙的雌性老鼠身體中的MCF-7腫瘤生長的影響作用

Tumor Size (cubic mm)
腫瘤的大小(立方毫米)
Days Post-Inoculation	Control	0.5% Ginseng 飲用含有0.5%	1% Ginseng
癌細胞二次孕育的天數	控制組 （沒有飲用含有野參萃取物的液體）	野參萃取物的液體	飲用含有1%野參萃取物的液體
11天	37.5 立方毫米	37 立方毫米	34.5 立方毫米
18天	62.5 立方毫米	61立方毫米	40.5 立方毫米
25天	96.5 立方毫米	93.5 立方毫米	46 立方毫米
32天	113 立方毫米	112 立方毫米	61 立方毫米

對美國野參抗癌靈實驗報告原文

                             ----墨菲教授（美國南伊利洛州醫科大學）

Effects of American Ginseng and Chinese Herbal Powder, Alone and in Combination, on Human Breast Cancer MCF-7 Cells In Vitro.

MURPHY, Laura L.,Ph.D.; ZONG, Wuying; RICE, Jennifer A..
Department of Physiology, Southern Illinois University School of Medicine,
Carbondale , IL 62901

In traditional Chinese medicine, herbals have been utilized in the treatment regimen for human cancers. Ginseng is often included in herbals used for the prevention and treatment of cancer. We have recently reported that a water extract of cultivated American ginseng root (Panax quinquefolium) inhibited the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells in culture. In the current study, we examined the effects of a Chinese herbal powder (CHP; a gift from American WildSenergy, Inc., New York, NY) currently used in the treatment of human cancers, on MCF-7 cell proliferation. The effects of CHP alone and in combination with American ginseng root extract were studied. The CHP and 10 year-old woods grown ginseng root powder (American WildSenergy, Inc.) were individually water-extracted, lyophilized, and stored at -20. MCF-7 cells were plated (5x104 cells) in DMEM media supplemented with 10% FBS and antibiotics and 24h later were treated with ginseng extract or CHP in doses of 0.01-2.5 mg/ml. Cells were treated every 2 days and, on day 6, trypsinized cells were counted using a hemocytometer. Treatment with either CHP or ginseng alone produced a significant dose-dependent decrease in MCF-7 cell proliferation when compared to media-only controls. The IC50 for CHP and ginseng was 0.4 and 0.5 mg/ml, respectively. Whereas doses of 1 mg/ml or higher of CHP killed all cells, ginseng did not appear to have cytotoxic effects at any dose. In the next study, MCF-7 cells were treated as described above with a wide range of ginseng doses (0.01-1.0 mg/ml) in combination with 0.4 mg/ml CHP. The IC50 for the ginseng+CHP combination was 0.02 mg/ml. These results indicate that either ginseng or CHP alone inhibits MCF-7 cancer cell proliferation, but that a combination of ginseng with CHP dramatically increases the potency of either compound alone. Thus, co-administration of American ginseng with cancer therapeutic drugs or herbals may increase the overall efficacy of treatment.

Letter from Prof. Murphy: When combining wild ginseng and CHP,ie. the wildsenergy capsules, the potency was increased by 25 times. Also, older ginseng root (10 years or more ) is twice as potent as the 5 year old root.

Laura L Murphy, Ph.D..
Department of Physiology
School of Medicine
Southern Illinois University
Carbondale, IL62901~6512
July 20, 2001

Mr. Lam
Allentown, PA 18103

Dear Mr. Lam:

I would like to thank you for sending me the fine samples of American wild ginseng and also the Chinese herbal powder for testing. I am sending you the results of the studies I have finished. We powderized the different ginseng roots, prepared individual water extracts of the powdered root, freeze-dried them, and individually tested them in our cancer cell bioassay. We prepared several different doses of each ginseng extract and treated human breast cancer MCF-7 cells with the extract for 6 days. You will see in the first graph that all of the extracts decreased the number of proliferating cancer cells, relative to control, non-treated (vehicle) cells, in a nice, dose-related manner. The IC50 value (dose of ginseng that inhibits cell proliferation by 50%) for the 18 yr. old root revealed that it was 2X more potent than the 5 yr. old root and that the 10 yr. old root was approximately as potent as the 18 yr. old root. I have tested several other roots that were sent to me and have found the results with your 5 yr. old root to be consistent with other 5-7 yr. old, woods grown roots. I have received only one other wild root sample that was > 18 yr. old and it was a little more potent than your sample. It was a root from Kentucky.

Regarding the Chinese herbal powder (CHP) study, we water-extracted the powder and performed the same kind of dose-response experiments as described above. As you can see on the second graph, we obtained another nice dose-response curve that demonstrated that the CHP had an IC50 of 0.4 mg/ml. At the highest dose of CHP, almost all of the cancer cells were dead. When we combined the American wild ginseng (10 yr. old root) with the CHP (0.4mg/kg), the potency was increased 25X. Therefore, a combination of CHP and ginseng very dramatically increased the potency of either compound alone. Furthermore, you will see that the highest ginseng dose tested, when combined with CHP, actually killed all of the cells. It is interesting that the ginseng just stops cells from proliferating (cytostatic), whereas the CHP was cytotoxic, in that it actually killed cells. This is the method by which most of the known cancer chemotherapy drugs act. I have enclosed an abstract describing this study that I am also sending to Dr. Li and Mr. Zeng.

Please let me know if I can provide you any more information.

Sincerely,

Laura L. Murphy, Ph.D. Associate Professor of Physiology

Laura L Murphy, Ph.D..